ligation of a 8kb fragment

[Ned Mantei]

In article <belville-2410961404200001 at cherubin.ens.fr>,
belville at wotan.ens.fr (Corinne BELVILLE) wrote:

> I would like to realize a ligation of a fragment whom the size is 8kb. My
> ligation failed in vector pGEM-T (at 15°C).I put 5 or 20 more inserts than
> plasmid.

See Revie, Smith, and Yee, Nucleic Acids Res. 16:10301-10321, 1988,
"Kinetic analysis for optimization of DNA ligation reactions", especially
table 3.

-- 
Ned Mantei
Dept. of Neurobiology, Swiss Federal Institute of Technology
CH-8093 Zurich, Switzerland
mantei at neuro.biol.ethz.ch   Fax: +41-1-633-1046