T/A cloning: efficient enough for library-making?

Minsoo Yoon ms.yoon at auckland.ac.nz
Fri Oct 25 10:48:23 EST 1996


In my case, it was efficient enough for my PCR library.
I could get 20,000 something colonies per standard electroporation, most
of them had inserts with variable sizes, among them 150 - 250 were
positives when probed.



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