How many bases overlap in recombinant PCR?

Zhengyang Wu zywzyw at lysator.liu.se
Thu Oct 24 10:57:48 EST 1996


I failed to do PCR-based recombination when the overlapping was less than
12 bp. When I made new oligoes and increased the overlapping to 18 bp, it 
worked fine. 

No idea to save such little amount of money: if you have to optimize the PCR
recombination with shorter oligoes, it will cost much more than just 
make a long one from the beginning. They also save your frustration!

regards,

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Zhengyang Wu, e-mail: zywzyw at lysator.liu.se
Vastanagatan 20-215, S-582 35, Linkoping, Sweden



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