I failed to do PCR-based recombination when the overlapping was less than
12 bp. When I made new oligoes and increased the overlapping to 18 bp, it
worked fine.
No idea to save such little amount of money: if you have to optimize the PCR
recombination with shorter oligoes, it will cost much more than just
make a long one from the beginning. They also save your frustration!
regards,
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Zhengyang Wu, e-mail: zywzyw at lysator.liu.se
Vastanagatan 20-215, S-582 35, Linkoping, Sweden
