Are his-tagged proteins active???
espinoza at cgl.ucsf.edu
Thu Oct 24 13:22:54 EST 1996
>In article <325CFE64.7D30 at kemi.aau.dk>, Kasper Engkilde
><kasper at kemi.aau.dk> wrote:
>> Thank you for you interest...
>> I'm considering starting on a project where I will put a His-tag on my
>> protein(s) but are conserned that I can't trust it to be active. My
>> question is therefore:
>> What experience do you have with the activity of his-tagged proteins???
>> Good and bad are of cause equally welcome!
>> On before hand - Thank you for your time and help.
>> Sincerely Kasper Dreyer Engkilde
It's worked great for me. (n=5, personally) In general,
we've only had one inactive 6his-fusion, and that cleared
up when we moved the tag to the other end of the protein. -Hernan
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