I have always been taught to wash cells with PBS before using trypsin
to wash off FCS. However, I was in a rush today, had forgotten to
heat up the PBS and used trypsin without washing. The cells split
fine.
So my question, is there a reason this is bad science?
Ruth.
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Ruth McMahon, PhD., Tel: 706-2815
Biotechnology Centre, Fax: 269-2016
University College Dublin,
Belfield,
Dublin 4,
Ireland.
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