High G/C PCR

brett brett at BORCIM.WUSTL.EDU
Thu Oct 24 10:19:46 EST 1996


>Hello:
>can anyone give me some advises for my PCR on a piece of DNA that contains
>about 70% G/C? I have a lot of troubles to make the PCR working.
>thank you very much
>X. Jia,
>Xjia at uci.edu

I've heard 1M (yes, molar) betaine will minimize G/C effects and still give
good amplification. This is from a local PCR expert, but I haven't tried it
myself.


Brett Lindenbach
    
Program in Immunology                              
Washington University - St Louis                  
brett at borcim.wustl.edu                             

"I own my own pet virus. I get to pet and name her." - Cobain




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