How much PCR template?

Barry Mook barry at sccs.swarthmore.edu
Thu Oct 24 10:05:06 EST 1996


Hi fellow biologists,

   My PCR's aren't working very well.  I was wondering whether I could be
using too much DNA....easy enough to test, but what generally is the
problem with using too much template in a reaction.   What should you
see?  How come it doesn't work?

Barry
barry at sccs.swarthmore.edu



More information about the Methods mailing list