How much PCR template?

Barry Mook barry at
Thu Oct 24 10:05:06 EST 1996

Hi fellow biologists,

   My PCR's aren't working very well.  I was wondering whether I could be
using too much DNA....easy enough to test, but what generally is the
problem with using too much template in a reaction.   What should you
see?  How come it doesn't work?

barry at

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