: can anyone give me some advises for my PCR on a piece of DNA that contains
: about 70% G/C? I have a lot of troubles to make the PCR working.
: thank you very much
: X. Jia,
:Xjia at uci.edu
High G/C? No problem.
Just add 15% glycerol in your PCR master mix, it'll work. I once tried
everything to amplify a high G/C bacterial DNA, the glycerol worked best.
The theory: glycerol can lower the anealing temperature and makes DNA
denature easier?
Alex Chang
Pathology
University of British Columbia
achang at hivnet.ubc.ca
