Can ssDNA transform competent cell ?

wliu at receptor.pharm.uwa.edu.au wliu at receptor.pharm.uwa.edu.au
Fri Oct 25 21:17:47 EST 1996


Hi netters,
Does anybody know that if the purified ssDNA (isolated from bacteria
containing phagemid after infection of helper phage RO408) can
transform competent cells and recover the dsDNA replication mode?
I recently got such result when I transformed competent ES1301 cells
with ssDNA  as a control. The colonies express the resistance to
tetracycline which is the gene located in the ssDNA. It is appreciated
if anyone can give me any ideas an\bout this.
Many thanks.
Williams





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