SYBR II problems

[Manuel J. Matos]

I am having problems with SYBR Green II (FMC) for RNA staining. After
running the RNA agarose gels, I immerse them in 10 mM sodium phosphate
buffer, pH 6.8 and a 1:5,000 SYBR Green II (as indicated by FMC). Bands
become clearly visible over a transilluminator only after 4 hours of
incubation, instead of the 30 minutes mentioned by FMC. Hints anyone?

* * * * * * * * * * * * * * * * * * * * * * * * * * * * *
* Manuel J. Matos (Manolo) -- mjmgmatos at gemini.ci.uc.pt *
* Departamento de Bioquimica *  Universidade de Coimbra *
* Aptd.  3126  *  P - 3000  Coimbra  *  P O R T U G A L *
* tlf. +351-39-22174 *Macintosh user* fax +351-39-33827 *
* * * * * * * * * * * * * * * * * * * * * * * * * * * * *