creation of blunt end DNA

R. Woodward rw200 at cus.cam.ac.uk
Fri Oct 25 11:28:24 EST 1996


Dear all

I want to clone a cDNA behind a fusion partner in a yeast two hybrid vector.
Obviously I have to be careful about maintaining the reading frame. The
only way I can do this is to cut the vector with Sfi I and then make this a 
blunt end to then allow me to blunt end ligate my cDNA in. My question is
can I use mung bean nuclease to create my blunt end? If so how accurate 
is MBN at stopping once it comes to the double stranded DNA. Also are there
any alternatives?

Thanks very much

Robert

rw200 at cus.cam.ac.uk

i
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