In article <326FE5F8.6030 at usfca.edu>, chihara at usfca.edu wrote:
> John Watson wrote:
> >
> > Barney P. Crum wrote:
> > >
> > > I'm currently doing northerns using a 35mer oligo to probe for a specific
Have a look at Trayhurn et al (1995) in Electrophoresis 16, 341-344.
"Rapid chemiluminescent detection of mRNAs on Northern blots with
digoxigenin end-labelled oligonucleotides"
This paper gives all the details for hybridization with oligos etc.
--
Derek Parsonage Wake Forest University
Dept. of Biochemistry Medical Center
dp at invader.bgsm.wfu.edu Winston-Salem NC USA