IUBio

oligos vs others

Derek Parsonage dp at invader.bgsm.wfu.edu
Fri Oct 25 08:52:01 EST 1996


In article <326FE5F8.6030 at usfca.edu>, chihara at usfca.edu wrote:

> John Watson wrote:
> > 
> > Barney P. Crum wrote:
> > >
> > > I'm currently doing northerns using a 35mer oligo to probe for a specific

Have a look at Trayhurn et al (1995) in Electrophoresis 16, 341-344.
"Rapid chemiluminescent detection of mRNAs on Northern blots with
digoxigenin end-labelled oligonucleotides"
This paper gives all the details for hybridization with oligos etc.

-- 
Derek Parsonage                        Wake Forest University
Dept. of Biochemistry                     Medical Center
dp at invader.bgsm.wfu.edu                 Winston-Salem NC  USA                                                               



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