High G/C PCR

[Jill Hollenbach]

I agree, although I generally use 20% glycerol. Works great.
            Jill


> : can anyone give me some advises for my PCR on a piece of DNA that contains
> : about 70% G/C? I have a lot of troubles to make the PCR working.

> Just add 15% glycerol in your PCR master mix, it'll work. I once tried