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High G/C PCR

Jill Hollenbach jillh at mendel.berkeley.edu
Fri Oct 25 07:13:50 EST 1996

I agree, although I generally use 20% glycerol. Works great.

> : can anyone give me some advises for my PCR on a piece of DNA that contains
> : about 70% G/C? I have a lot of troubles to make the PCR working.

> Just add 15% glycerol in your PCR master mix, it'll work. I once tried

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