In article <326DAA54.764E at bric.postech.ac.kr>,
mhlee at BRIC.POSTECH.AC.KR (Min-Ho Lee) wrote:
>Hello!
>I have a problem in purification of an insect virus.
>When viral particles were collected (as a white band) in sucrose
>gradients (30%/60%), I transferred the band to a new ultracentrifuge
>tube.
>Then, I spun the tube at 100,000g for 1 hr. But, I have no get any
>pellet. I thought that viruses have no spun down because of
>sucrose buoyancy.
>So, I allowed the solution at 4oC, overnight, stirred it roughly and
>ultracentrifuge at 100,000g for 1 hr. But, I have no any pellet, too!
>>Is there anyone know about it?
>Why virus particles were spun down?
>What can I do for purification of the virus particles?
>>Your Sincerely, Min-Ho Lee
Are you sure the "white band" was composed of virus?
Did you dilute the sucrose to reduce the concentration to <10% before trying to
sediment the virus? It takes a lot of virus to get a visible pellet.
RMVF
