I'm currently DIG labelling a 280bp cDNA fragment (extracted from
agarose gel using QIAEX from Qiagen) by random priming, which was later
to be used in Southern hybridization.
However, the labelling efficiency was very low. Only about 100ng of DIG
labeled probe obtained after a 20hrs reaction from 500ng template.
(Determined by using a spot test and compare with the control provided
in the kit)
Can any body help..??? Any suggestion and discussion are greatly