DIG labelling by random priming...
lumdicks at netvigator.com
Sat Oct 26 21:51:12 EST 1996
I'm currently DIG labelling a 280bp cDNA fragment (extracted from
agarose gel using QIAEX from Qiagen) by random priming, which was later
to be used in Southern hybridization.
However, the labelling efficiency was very low. Only about 100ng of DIG
labeled probe obtained after a 20hrs reaction from 500ng template.
(Determined by using a spot test and compare with the control provided
in the kit)
Can any body help..??? Any suggestion and discussion are greatly
More information about the Methods