we just got some P1 and cosmids, both with kanamycin resistance, we tried
the usual wizard plasmid minipreps, but got a very low yield, and too much
degradation (smears). For one of these, we were told to induce with IPTG,
but it didn't do much.
Any tricks we're not aware of?
pls reply directly to me,
i don't read this newsgroup.