Help: P1/cosmid DNA prep.

Marc_Champagne Marc_Champagne at cellbio.duke.edu
Sat Oct 26 22:28:07 EST 1996


Hi,

we just got some P1 and cosmids, both with kanamycin resistance, we tried
the usual wizard plasmid minipreps, but got a very low yield, and too much
degradation (smears).  For one of these, we were told to induce with IPTG,
but it didn't do much.

Any tricks we're not aware of?

Thanks,

Marc

pls reply directly to me,
i don't read this newsgroup.



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