IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Sequencing GC-Rich DNA

Malia Fullerton S.M.Fullerton at durham.ac.uk
Fri Oct 25 05:00:40 EST 1996


We are having considerable difficulty obtaining readable sequence from
several different human nuclear genes.  I suspect that GC-richness and/or
secondary structure may be influencing sequencing efficiency, as primers
placed at different locations give the same poor-quality sequence, and we
are using (what has been with other loci) ample quantities of
single-stranded template.

What are the best (or at least most commonly used) methods for addressing
this sort of sequencing problem?  References appreciated.




More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net