Acetyl CoA for CAT

ER at total.net ER at total.net
Fri Oct 25 22:42:34 EST 1996


We've stored Acetyl-CoA dissolved at -80 for over many many months and
it seems to still be working fine for CAT assays.
-ER

Martin Hughes <mhughes at fhs.csu.McMaster.CA> wrote:


>Hi everyone!
>I recently started doing CAT assays from transfected cells.  The method I 
>am using is basically that as stated in Sambrook et al., using the 14C 
>-chloramphenicol and TLC assay procedure.
>The assay calls for Acetyl CoA, which, according to the method, should be 
>made fresh each time.
>This stuff seems to be pretty expensive, and the amount one gets is 
>tracingly small.
>I have recently found that the 100mg quantity I bought (at CA$300+), 
>which should have lasted about 50 sets of experiments, only lasted for 
>about 10 - probably due to wastage in weighing out/stuck to the sides of 
>the bottle.
>Has anyone found a cure for this problem?
>Is it possible to dissolve the whole bottle and store it at -70, or under 
>Argon, or some such thing?
>Also, I would greatly appreciate it if anyone is aware of any cheap(er) 
>sources of the chemical.

>Cheers, and thanks in advance,

>Martin

>Martin Hughes
>Department of Biochemistry
>McMaster University
>Hamilton, ONTARIO, Canada.






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