We've stored Acetyl-CoA dissolved at -80 for over many many months and
it seems to still be working fine for CAT assays.
Martin Hughes <mhughes at fhs.csu.McMaster.CA> wrote:
>I recently started doing CAT assays from transfected cells. The method I
>am using is basically that as stated in Sambrook et al., using the 14C
>-chloramphenicol and TLC assay procedure.
>The assay calls for Acetyl CoA, which, according to the method, should be
>made fresh each time.
>This stuff seems to be pretty expensive, and the amount one gets is
>I have recently found that the 100mg quantity I bought (at CA$300+),
>which should have lasted about 50 sets of experiments, only lasted for
>about 10 - probably due to wastage in weighing out/stuck to the sides of
>Has anyone found a cure for this problem?
>Is it possible to dissolve the whole bottle and store it at -70, or under
>Argon, or some such thing?
>Also, I would greatly appreciate it if anyone is aware of any cheap(er)
>sources of the chemical.
>Cheers, and thanks in advance,
>Department of Biochemistry
>Hamilton, ONTARIO, Canada.