extracting very very dilute DNA

Jim Hutchins hutchins at fiona.umsmed.edu
Mon Oct 28 20:55:25 EST 1996


Christine Wulff-Strobel (cwulff at pop.uky.edu) wrote:
: I am looking for a way to extract DNA from single celled organisms which 
: may be contaminating animal feed samples. Since the density of these 
: organisms is similar to the feed particles themselves (when ground up and 
: solubilized in water) simple centrifugation is not an option. Filtration 
: has not been successful either because the feed clogs the filters.

:      Does anyone out there have experience with or a reference for total 
: DNA extraction/harvest from extremely dilute aqueous samples?

:      Thanks in advance for you responses.

--
Jim Hutchins
Assoc Prof Anatomy, Asst Prof Neurology (Research), Univ Mississippi Med Ctr
http://fiona.umsmed.edu/~hutchins/    ***    E-mail:   hutchins at umsmed.edu
``Battles between academics are so bitter because so little is at stake.'' 
  Robert Maynard Hutchins [no relation]



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