Double-transfection (stable) of COS-7 cells
juan_vilar at bchm.biochem.duke.edu
Mon Oct 28 12:55:41 EST 1996
In article <slrn574ova.itt.aiyar at ebv.oncology.wisc.edu>,
aiyar at ebv.oncology.wisc.edu wrote:
ogy 1995 Feb;69(2):1280-3
> ......There is one other point I wish to emphasize. There is no arbitrary
> "maximum" copy number of oriP plasmids that can be maintained within cells.
> The number of oriP plasmids within any particular clone is HIGHLY
> dependant upon the number of copies of the oriP plasmid initially
> introduced into the cell, i.e the efficiency of transfection.
> Ashok Aiyar, Ph.D.
> Department of Oncology email: aiyar at ebv.oncology.wisc.edu
> University of Wisconsin-Madison tel: (608) 262-6697
Kevin: I think that you could try to produce stable transfectants by
co-transfection of COS cells with both oriP plasmids at once (three
different experiments with different ratios plasmid1/plasmid2) and select
for G418/hygromycin resistant cells.
Hope this helps,
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