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Double-transfection (stable) of COS-7 cells

hill juan_vilar at bchm.biochem.duke.edu
Mon Oct 28 12:55:41 EST 1996

In article <slrn574ova.itt.aiyar at ebv.oncology.wisc.edu>,
aiyar at ebv.oncology.wisc.edu wrote:
ogy 1995 Feb;69(2):1280-3

> ......There is one other point I wish to emphasize.  There is no arbitrary
> "maximum"  copy number of oriP plasmids that can be maintained within cells.
> The number of oriP plasmids within any particular clone is HIGHLY
> dependant upon the number of copies of the oriP plasmid initially 
> introduced into the cell, i.e the efficiency of transfection.
> later,
> Ashok
> -- 
> Ashok Aiyar, Ph.D.
> Department of Oncology            email: aiyar at ebv.oncology.wisc.edu
> University of Wisconsin-Madison                  tel: (608) 262-6697

Kevin: I think that you could try to produce stable transfectants by
co-transfection of COS cells with both oriP plasmids at once (three
different experiments with different ratios plasmid1/plasmid2) and select
for G418/hygromycin resistant cells.

Hope this helps,

Juan Perez-Vilar

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