Distinguishing mutant sequence in heterozygote?
gmj at waikato.ac.nz
Mon Oct 28 16:16:49 EST 1996
I am analysing tumours for p53 mutation type using PCR and automated
sequencing. My electropherograms of an exon five sequence show concurrent
peaks - indicative of a mutation against a wildtype background?- at places
along the sequence.
I would be grateful for suggestions of an efective methodology for the
distinguishing of mutant sequences (represented in approx. 10% of cells in
each sample) against a wildtype background.
Thanks in advance,
1st yr MSc student
Uni. of Waikato
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