cell clumping after electroporation

Dima Klenchin klenchin at facstaff.wisc.edu
Wed Oct 30 00:08:30 EST 1996


In article <5557s5$j8j at mserv1.dl.ac.uk>, "FABIAN MOEBIUS" <Fabian.Moebius at uibk.ac.at> wrote:
#Various people in our lab now experience with different types of frozen
# competent cells that 
#after electroporation and 1 hour growth at 37 degres the cells are 
#not homogenous but show sticky filaments as seen with precipitated high 
#molecular weight DNA (looks like alien worms). Did anybody experience this
# before? We suspect 
#that this must be a problem of cuvettes, medium added after EP or 
#eprouvettes used for incubating the cells before plating.

That's OK. Electroporation kills and lyses many cells. What you see IS 
DNA. As long as efficiency is good, no reason to worry. If electoporation 
is unefficient, it might be that you are killing too much. Then check the 
conditions. 

- Dima



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