This inquiry is for a colleague, so I may not have all the facts at my
finger tips. My colleague wants to use DS DNA as a substrate in a type
of ELISA set up. The question is, how much DNA from a known starting
amount is bound? Obviously, the unbound DNA is quantitated, but how?
I guess the best (most sensitive) method would be to use radio- or
fluorochrome labeled DNA. However, the new method is being set up to
avoid using radioactivity and there is no fluorometer available.
I was thinking about maybe DIG labeled DNA, but there may be problems
associated with the DIG interfering further down the line. Anyone have
any better ideas? I think the amount of DNA would be in the