transfection cells of neural origin

Andrea LeBlanc mdal at musica.mcgill.ca
Tue Oct 29 07:03:28 EST 1996


In article <54nptl$6vm at sun0.urz.uni-heidelberg.de>,
un691cs at genius.embnet.dkfz-heidelberg.de (z) wrote:

> What is the preferred method for transfection of
> PC-12, C6, Lan-1 and B49 cells ? anybody out 
> there transfecting any of these cell lines ?
> 
> z

You can use lipofectin with Lan1 using 5 µg DNA, and 15 µg lipofectin in
50µl OPTI-MEM. I don't know about the transfection efficiency but I got
stables that amplified very quickly. I don't know about the other cell
lines.
Good luck
Andrea.

-- 
Andrea LeBlanc



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