Removal of carbohydrates from DNA preps

Claudia egreif at cc.umanitoba.ca
Tue Oct 29 11:20:17 EST 1996


In article <326B2C8D.5554 at credit.erin.utoronto.ca>, Farid jalali
<fjalali at credit.erin.utoronto.ca> wrote:

> Can anyone offer suggestions on how to remove carbohydrates from 
> cyanobacterial genomic DNA preps. Any help would be greatly appreciated.
> 
> Thanks
> 
> Farid Jalali
> Dept. of Botany
> Erindale College
> University of Toronto
> Canada

You could run your DNA on a CsCl gradient or you could try CTAB
extraction. Make your DNA solution .7M Nacl, add 1/10 vol of 10% CTAB, .7M
NaCl, mix, heat at 65°C for 20 min, extract with an equal vol of
chloroform. Collect aqueous phase, carbohydrates should be at the
interface.

-- 
Hey, hey, hey, hey! It was the DNA.
Hey, hey, hey, hey! That made me this way.



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