My group works on mammalian cell engineering. I am looking for
techniques/reagents to increase cloning efficiency of the PEER cell
line. I have already tried conditionned medium, high serum
concentration and even IL-2, with limited success.PEER is an acute
lymphoblastic leukemia of T cell type.
We have many variants of it expressing many interesting genes, but
most of them have a very poor cloning efficiency.
Please reply by email, as we don't subscribe to this list.
Antoine W. Caron
Biotechnology Research Institute/NRC