I am performing SSCP analysis on PAGE gels for mutation detection.
The gels take a few hours to run at room temperature, but several hours
at the coldroom.
I wonder if I can increase the running time (and decrease the voltage)
without significant reduction in sensitivity. I intend to run the gels
overnight at the approptiate voltage.
I would be grateful if anyone had any information for that?
Dept. Genetics, Development and Molecular Biology
School of Biology
Aristotle University of Thessaloniki
e-mail: aris at serifos.bio.auth.gr