Optimal running time for SSCP

Aris Astrinidis aris at serifos.bio.auth.gr
Thu Oct 31 02:44:45 EST 1996


Dear coleagues,
I am performing SSCP analysis on PAGE gels for mutation detection.
The gels take a few hours to run at room temperature, but several hours
at the coldroom.
I wonder if I can increase the running time (and decrease the voltage)
without significant reduction in sensitivity. I intend to run the gels
overnight at the approptiate voltage.

I would be grateful if anyone had any information for that? 

Sincerely,
Aris Astrinidis
Dept. Genetics, Development and Molecular Biology
School of Biology
Aristotle University of Thessaloniki
GR-54006 Thessaloniki
GREECE

Tel. +30-31-998378
FAX. +30-31-998374
e-mail: aris at serifos.bio.auth.gr



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