multi-bands on microsatellites

Shelley Cole shelley at DARWIN.SFBR.ORG
Wed Oct 30 15:47:52 EST 1996


John,
Check out these papers and references therein:
	Mellersh and Sampson. 1993. BioTechniques 15;4:582-584.
	Litt, Hauge, and Sharma. 1993. BioTechniques 15;2:280-284.
They explain some of the origins of stutter bands, and present some of 
the strategies used to decrease their appearance.

Shelley

On Wed, 30 Oct 1996, John Korte wrote:

> Greetings,
> I've just recently began microsatellite amplifications in our lab using 
> 33P-dCTP and the gels (6% sequencing)  have 3-5 bands in the area of the 
> product.  Could someone out there explain this "stutter" effect to me?
> Thanks, 
> John
> 
> 



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