multi-bands on microsatellites
shelley at DARWIN.SFBR.ORG
Wed Oct 30 15:47:52 EST 1996
Check out these papers and references therein:
Mellersh and Sampson. 1993. BioTechniques 15;4:582-584.
Litt, Hauge, and Sharma. 1993. BioTechniques 15;2:280-284.
They explain some of the origins of stutter bands, and present some of
the strategies used to decrease their appearance.
On Wed, 30 Oct 1996, John Korte wrote:
> I've just recently began microsatellite amplifications in our lab using
> 33P-dCTP and the gels (6% sequencing) have 3-5 bands in the area of the
> product. Could someone out there explain this "stutter" effect to me?
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