IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Selective PCR amplification

John Watson watson_j at bms.com
Wed Oct 30 13:56:44 EST 1996

Doug Johnson wrote:
> We are trying to clone several closely related sequences via PCR
> using the same primers. The products are different sizes, from 300 to
> 600 bp. When we amplify and clone, we have to screen many recombinant
> before we can find the smaller ones. Is it possible to control the PCR
> conditions e.g., by lowering the time of extension, in order to selectively
> amplify the smaller products?
> Any suggestions greatfully considered.

Is there any reason why you cannot gel-purify the fragment(s) of interest and 
then clone?

John Watson
Bristol-Myers Squibb Co.
watson_j at bms.com
"If you're not part of the solution, you're part of the precipitate."

More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net