Selective PCR amplification

John Watson watson_j at bms.com
Wed Oct 30 13:56:44 EST 1996


Doug Johnson wrote:
> 
> We are trying to clone several closely related sequences via PCR
> using the same primers. The products are different sizes, from 300 to
> 600 bp. When we amplify and clone, we have to screen many recombinant
> before we can find the smaller ones. Is it possible to control the PCR
> conditions e.g., by lowering the time of extension, in order to selectively
> amplify the smaller products?
> 
> Any suggestions greatfully considered.

Is there any reason why you cannot gel-purify the fragment(s) of interest and 
then clone?

---------------
John Watson
Bristol-Myers Squibb Co.
watson_j at bms.com
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