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seq and betaine

Dave B dboyd at cc.umanitoba.ca
Wed Oct 30 13:22:07 EST 1996

Read the article in NAR a few issues ago about sequencing with Sequenase
and using betaine in the termination mixes to eliminate pausing. Looks
excellent as a standard modification. My question is the recipe for the rx
buffer. They call it TDMN and the recipe is: 280 mM TES, 200 mM NaCl, 120
mM HCl, and 50 mM DTT. What is TES? I am assuming it contains the MgCl2
needed for the rxns.


Dave B

Hey, hey, hey, hey! It was the DNA.
Hey, hey, hey, hey! That made me this way.

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