We use high concentration (2mg/ml) of proteinase K and then ethanol
precipitation to purifiy DNA from cellular extracts. After that some
enzymes doesn't work efficiently, probably due to the rest of
proteinase K in the precipitate, I need help for a protocol in order
to inactivate proteinase K efficiently, other than phenol extraction.
ijiwaru at wheel.dcn.davis.ca.us wrote:
Agnes.Cordonnier at esbs.u-strasbg.fr (Agnes Cordonnier (UPR9003-CNRS))