Inactivation of Proteinase K

Masato Furuichi masato at esbs.u-strasbg.fr
Wed Oct 30 12:38:13 EST 1996


Hi,

We use high concentration (2mg/ml) of proteinase K and then ethanol 
precipitation to purifiy DNA from cellular extracts. After that some 
enzymes doesn't work efficiently, probably due to the rest of 
proteinase K in the precipitate, I need help for a protocol in order 
to inactivate proteinase K efficiently, other than phenol extraction.
ijiwaru at wheel.dcn.davis.ca.us wrote:
> 

Thanks

Agnes.Cordonnier at esbs.u-strasbg.fr (Agnes Cordonnier (UPR9003-CNRS))



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