Pigments are coextracted with RNA....

[Hong Seokjin]

When I was isolating RNA from leech(annelid) with hot-phenol method,
I couldn't remove black-colored pigments from RNA. I treated proteinase K,  
however, the pigments were remained after treatment. When I used
LiCl, the yield of isolated RNA was too low. As I think, pigment might 
bind to RNA when the leech was homogenized.

How can I remove pigments (or ?) from RNA?  
  


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  Hong Seok Jin               E-MAIL : sjhong at bioneer.kaist.ac.kr
  Dept. of Biological Sciences            BioResource Laboratory
  Korea Advanced Inst. of Sci. and Tech. ( KAIST )
  Voice : +82-42-869-5612$CALL$$
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