Best way to clean cuvettes?
Achim Recktenwald, PhD
achim at ibex.ca
Wed Oct 30 08:53:43 EST 1996
Matthew Parker wrote:
>
> todd andrew johnson wrote:
> >
> > How often does your lab clean your cuvettes after each sample, set
> > of samples, periodically?
> >
> I'm talking about between runs; we do kinetic assays in the cuvettes,
> and trace amounts left over from the previous assay can mess things up.
>
> Matt
Especially when you are working with very hydrophobic substrates, or
enzymes very sensitive to changes in the surface tension. I worked for a
while with lipases. The washing of the cuvettes between the assays was
like a ritual, you forget one step and everything is messed up.
Achim
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