Matthew Parker wrote:
>> todd andrew johnson wrote:
> > How often does your lab clean your cuvettes after each sample, set
> > of samples, periodically?
> I'm talking about between runs; we do kinetic assays in the cuvettes,
> and trace amounts left over from the previous assay can mess things up.
Especially when you are working with very hydrophobic substrates, or
enzymes very sensitive to changes in the surface tension. I worked for a
while with lipases. The washing of the cuvettes between the assays was
like a ritual, you forget one step and everything is messed up.