Transfection of Drosophila Schneider cells

User please at use.adress.in.signature
Wed Oct 30 10:11:36 EST 1996


In article <genecutl-2110960149330001 at kosh-161b-1.berkeley.edu>,
   genecutl at mendel.berkeley.edu (gc) wrote:
>In article <5480ah$pk3 at sun168.rz.ruhr-uni-bochum.de>,
>please at use.adress.in.signature (User) wrote:
>
>* Hello !
>* Is there anyone who has experience in 
>* calcium-phosphat-coprecipitation of Drosophila Schneider cells S2 ?
>* The problem is, that I cannot detect any RNA of my transfected DNAs
>* by Nuclease S1 - Analysis: For example : VAI is a high expressed 
>* RNA Pol III - Gen, but the RNA is undetectable after RNA-Preparation
>* by the GIT-method.
>* Have you got any tips for a solution.
>* 
>* Thanks for your time !
>* 
>* Björn Sandrock
>* AG Benecke
>* Ruhr-Uni-Bochum, Germany
>
>Do your cells look OK after transfection?  When you do the precipitation,
>what does the DNA mix look like?  Have tried other assays, such as
>luciferase or beta-gal to double check?
>
>--gc

Hi again !
I think, that my problem might be a problem of the number of cells, which 
I have used the earlier times. There the cells looked very damaged. In my last 
experiment I used four times more cells and everything seemed ok with the 
cells. The RNA-Analysis is on the way!

Björn



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