Inactivation of Proteinase K

XIAO-MIN SU sumini at welchlink.welch.jhu.edu
Wed Oct 30 16:34:31 EST 1996



On Wed, 30 Oct 1996, Masato Furuichi wrote:

> Hi,
> 
> We use high concentration (2mg/ml) of proteinase K and then ethanol 
> precipitation to purifiy DNA from cellular extracts. After that some 
> enzymes doesn't work efficiently, probably due to the rest of 
> proteinase K in the precipitate, I need help for a protocol in order 
> to inactivate proteinase K efficiently, other than phenol extraction.
> ijiwaru at wheel.dcn.davis.ca.us wrote:
> > 
> 
> Thanks
> 
> Agnes.Cordonnier at esbs.u-strasbg.fr (Agnes Cordonnier (UPR9003-CNRS))
> 
> 
Try to incubate your samples at 100 degree for 10 min.

Xiao-min Su
Johns Hopkins University
Molecular Diagnostic Lab.



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