Inactivation of Proteinase K
sumini at welchlink.welch.jhu.edu
Wed Oct 30 16:34:31 EST 1996
On Wed, 30 Oct 1996, Masato Furuichi wrote:
> We use high concentration (2mg/ml) of proteinase K and then ethanol
> precipitation to purifiy DNA from cellular extracts. After that some
> enzymes doesn't work efficiently, probably due to the rest of
> proteinase K in the precipitate, I need help for a protocol in order
> to inactivate proteinase K efficiently, other than phenol extraction.
> ijiwaru at wheel.dcn.davis.ca.us wrote:
> Agnes.Cordonnier at esbs.u-strasbg.fr (Agnes Cordonnier (UPR9003-CNRS))
Try to incubate your samples at 100 degree for 10 min.
Johns Hopkins University
Molecular Diagnostic Lab.
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