On Wed, 30 Oct 1996, Masato Furuichi wrote:
>> We use high concentration (2mg/ml) of proteinase K and then ethanol
> precipitation to purifiy DNA from cellular extracts. After that some
> enzymes doesn't work efficiently, probably due to the rest of
> proteinase K in the precipitate, I need help for a protocol in order
> to inactivate proteinase K efficiently, other than phenol extraction.
>ijiwaru at wheel.dcn.davis.ca.us wrote:
>>Agnes.Cordonnier at esbs.u-strasbg.fr (Agnes Cordonnier (UPR9003-CNRS))
>>Try to incubate your samples at 100 degree for 10 min.
Johns Hopkins University
Molecular Diagnostic Lab.