Preparation of mitotic HeLa cells?

[Zhonglin Chai]

Hi there,

I am looking for:
1).protocals to prepare mitotic HeLa cells;
2).information of cell cycle times (each phase) of HeLa
cells.

I am trying to isolate mRNA from mitotic HeLa cells. I
would appreciate any prototocals and suggestions to prepare
and harvest mitotic cells. I am thinking of doing double
block of the cells at G1/S border using thymidine and then
release the cells by washing, allowing majority of the cells
to enter M phase at the same time. Information of the time
(hours) that each phase of HeLa cells would take would be
very helpful.

Please email me your reply to:
zchai at cobra.path.monash.edu.au




Dr. ZhongLin Chai
____________________________________________
Department of Pathology and Immunology
Monash University Medical School
Alfred Hospital
Commercial Rd, Prahran, Victoria, AUSTRALIA
Telephone: (61 3) 9276 2698 (lab)
           (61 3) 9276 2696 (office)
           (61 3) 9388 1205 (home)
Fax:       (61 3) 9529 6484
email:     zchai at cobra.path.monash.edu.au
____________________________________________