I have a DNA extraction method that calls for Nonidet P-40 as the lysing
agent. However, I cannot find a source in Fisher, Sigma, or Pharmacia.
Does anyone have a source or catalog number for this detergent.
Also, is there a particular reason why one would use Nonidet P-40 over
SDS or Triton X-100 for lysing blood cells? Are there particular
applications where one agent is better than others, or are do they serve
the purpose equally well for all applications?
I appreciate your assistance.
rbright at mnw.net