Optimal running time for SSCP

John MacFarlane jmacfarl at hgmp.mrc.ac.uk
Thu Oct 31 08:45:48 EST 1996


On Wed, 30 Oct 1996, Aris Astrinidis wrote:

> Dear coleagues,
> I am performing SSCP analysis on PAGE gels for mutation detection.
> The gels take a few hours to run at room temperature, but several hours
> at the coldroom.
> I wonder if I can increase the running time (and decrease the voltage)
> without significant reduction in sensitivity. I intend to run the gels
> overnight at the approptiate voltage.
 
Aris,

We run our SSCP gels (35cm long, 1mm thick) overnight at 5 watts as
standard practice. Sensitivity is fine (95% mutation detection)and at this
rate fragments of approx 150bp + are still on the gel in the morning.

John MacFarlane





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