IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Optimal running time for SSCP

Nick Jacobsen jacobsen at cf.ac.uk
Thu Oct 31 06:21:16 EST 1996

Hi there,
        My lab routinely uses SSCP. We find that both at RT and in the 
cold room if you run a 6% gel in 0.5xTBE at 70v overnight you get very 
good sensitivity for a 270bp fragment. You say you are worried that 
sensitivity will decrease if you run it longer. I would say that running 
it for a few hours at RT would be a lot less sensitive due to the 
temperature of the gel at that sort of voltage.  

hope this helps



More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net