noguei01 at mcrcr6.med.nyu.edu wrote:
>> Hi there,
> I've been cloning from a lambda FixII genomic library. I have
> identified several clones and I will start mapping the inserts.
> I've been trying to obtain a restriction map for Lambda FixII from
> Stratagene with no success.
> Is there anyone that can help me with information of unique or not very frequent
> sites from these phage?
There is a map of FIX II in the back of the Stratagene catalog, and the company faxed and
emailed the sequence of the MCS when I requested it. I've included the MCS info below.
Stratagene bacteriophage l FIX-II Multiple Cloning Site
Sequence Range: 1 to 145
>NotI >XbaI >EcoRI
| | |
>XbaI >SacI| >SacI >SalI >XhoI >EcoRI | >XbaI |
| | | | T7 | | | | | |
| | | | -----------> | | | | | |
| * | | * | * * | * | * | | * | *
TCTAGAGAGC TCGCGGCCGC GAGCTCTAAT ACGACTCACT ATAGGGCGTC GACTCGAGAA GGAATTCTCT AGATCTAGAG
AGATCTCTCG AGCGCCGGCG CTCGAGATTA TGCTGAGTGA TATCCCGCAG CTGAGCTCTT CCTTAAGAGA TCTAGATCTC
>NotI >XbaI
| |
>XhoI >SalI >SacI| >SacI
| | T3 | | | |
| | <-------------------- | | 140
| * | * * * | | * | *
AATTCGGCTC GAGTCGACTC CCTTTAGTGA GGGTTAATTG AGCTCGCGGC CGCGAGCTCT CTAGA
TTAAGCCGAG CTCAGCTGAG GGAAATCACT CCCAATTAAC TCGAGCGCCG GCGCTCGAGA GATCT
See ya in the funny papers,
AJW
-----------------------
John Watson
Bristol-Myers Squibb Co.
watson_j at bms.com
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