ZnCl

[James Pearson]

In article <54t6pf$4n3 at isnews.csc.calpoly.edu> smccull at jurassic.fisher.calpoly.edu (The Kindly One) writes:
>From: smccull at jurassic.fisher.calpoly.edu (The Kindly One)
>Subject: ZnCl2
>Date: 26 Oct 1996 14:21:35 GMT

>Hello,

>The protocol I'm using calls for ZnCl2 in the micromolar
>range in my induction media(E. coli). I can't find any info
>on what kinds of stock to use, etc. in CP.

>ANy ideas? I initially made a 1M stock but it was cloudy and had
>precipitate on thawing.

>ANy suggestions greatly appreciated,
>scott

>-- 
>** Scott D. McCulloch(Bunny Lover)      | If we knew what we were doing,
>** smccull at jurassic.fisher.calpoly.edu  | it would not be called     
>** BioChemical Effects Research         | research, now would it?
>** http://www.calpoly.edu/~smccullo/    |


Hi Scott,

I have had similar problems with ZnCl2. I use a 100mM stock which does not 
exhibit the problems mentioned.  You do not need to freeze ZnCl2 solutions,
they are fine at RT.

				Good Luck

				James Pearson
				Molecular Microbiology and Immunology
				University of Missouri 
				c603670 at showme.missouri.edu