Concentrating proteins already in SDS sample buffer?
Dr E. Buxbaum
EB15 at le.ac.uk
Mon Sep 2 07:59:58 EST 1996
You may try ultrafiltration. Small volumes are handled in centrifugation
devices (Millipore, Centricon, Whatman too, I think), but larger volumes
(several ml) are better handled in stirred cells (Amicon). I would not
use any precipitation techniques, because it is usually difficult to get
proteins into solution again afterwards. I had this problem with both TCA
precipitation and Chloroform/Methanol, even Phenol/Ether resulted in most
of the protein staying in the wells during electrophoresis (but then I am
handling a 160 kDa membrane protein, yours may be less fastidious).
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