Remove SDS from protein preparation

Dr E. Buxbaum EB15 at le.ac.uk
Mon Sep 2 07:50:52 EST 1996


There are special detergent removal columns available (Pierce), but be 
warned: Flow through these columns is extremly slow. 

You may want to try the BCA method for protein determination, more 
sensitive than Lowry or Bradford and reasonably detergent tolerant. 
Biorad sell the chemicals as kit, but it is cheaper to by them 
seperately.




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