H7 PKC Inhibitor

Friedemann Weber fweber at SUN1.UKL.UNI-FREIBURG.DE
Mon Sep 2 04:19:20 EST 1996

Dear netters,

I want to inhibit PKC in cell culture by adding H7 (an isoquinoline 
sulphonamide) to the medium prior to transfection. Does anybody have 
any experience with this? Especially, I want to know at which 
timepoint it is best to add the inhibitor and how long the cells 
survive under H7. Are they susceptiple to a transfection at all?

Thanx in advance,

fweber at sun1.ukl.uni-freiburg.de(Friedemann Weber)

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