Westerns-blotting-BSA

Pascal Mertens Pascal.Mertens at fundp.ac.be
Wed Sep 4 00:44:07 EST 1996


In article (Dans l'article)
<Pine.SOL.3.91.960822141048.1166A-100000 at isnet>, Steve <smiles at bgsm.edu>
wrote (écrivait) :

> Hey all!
> 
> I'm doing a normal western blot, but our polyclonal antibodies seem to be 
> binding to many of the E. Coli proteins. It is possible that the 
> antibodies were raised to our protein, as well as any bacterial proteins 
> present in the blood during an infection in the rabbit.
> 
> Is there any protocol to add cell extract to the BSA blocking buffer, or 
> the primary antibodies, which will prevent all this binding?
> 
> Or is there a better way to block?
> 
> Thanks.
> 
> STEVE

You can try this latter one:
"Alternative method to remove antibacterial antibodies from antisera used
for screening of expression libraries" in: Biotechniques 19:28-30 (1995)

Pascal



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