Pascal.Mertens at fundp.ac.be
Wed Sep 4 00:44:07 EST 1996
In article (Dans l'article)
<Pine.SOL.3.91.960822141048.1166A-100000 at isnet>, Steve <smiles at bgsm.edu>
wrote (écrivait) :
> Hey all!
> I'm doing a normal western blot, but our polyclonal antibodies seem to be
> binding to many of the E. Coli proteins. It is possible that the
> antibodies were raised to our protein, as well as any bacterial proteins
> present in the blood during an infection in the rabbit.
> Is there any protocol to add cell extract to the BSA blocking buffer, or
> the primary antibodies, which will prevent all this binding?
> Or is there a better way to block?
You can try this latter one:
"Alternative method to remove antibacterial antibodies from antisera used
for screening of expression libraries" in: Biotechniques 19:28-30 (1995)
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