Transformation efficiency of supercoiled vs. relaxed?
mmgosink at facstaff.wisc.edu
mmgosink at facstaff.wisc.edu
Tue Sep 3 14:04:15 EST 1996
Dear Netters:
Does anyone know (or better yet have a reference) to the efficiency of
transformation of supercoiled DNA as compared to relaxed DNA? More
specifically if I have cells where I get 10^7 colonies per ug of a
standard supercoiled DNA, how many colonies will I get from 1ug of DNA
from a ligation?
Thanks in advance for any tips.
Mark
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