Transformation efficiency of supercoiled vs. relaxed?

mmgosink at facstaff.wisc.edu mmgosink at facstaff.wisc.edu
Tue Sep 3 14:04:15 EST 1996


Dear Netters:

	Does anyone know (or better yet have a reference) to the efficiency of
transformation of supercoiled DNA as compared to relaxed DNA? More
specifically if I have cells where I get 10^7 colonies per ug of a
standard supercoiled DNA, how many colonies will I get from 1ug of DNA
from a ligation?

	Thanks in advance for any tips.

Mark



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