RNA isolation Kits

C E Pical C.Pical at shef.ac.uk
Thu Sep 5 11:16:33 EST 1996


In article <mkhill-050996165229 at mg-a12-1-50.bio.usyd.edu.au>, 
mkhill at bio.usyd.edu.au says...
>
>
>
>I was just wondering if anyone was having success (bad experiences 
would be
>useful too!) using a kit, such as Ultraspec from Bresatec or RNeasy 
from
>Qiagen, to isolate RNA from plant tissue.
>
>Thanks



RNeasy from Qiagen worked fine for me and some other in the lab. 
However it leaves some DNA. But, I once did two successive elution and 
got much less DNA in the second elution step as compared to the first 
step, whereas the amount of RNA was almost the same in both elution 
steps.

C.




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