GENOMIC SOUTHERN: help with DNAseI Hypersensitivity (please!!!!!) (:

John Watson watson_j at
Thu Sep 5 08:35:27 EST 1996

Well, if I were you I would play with different hybridization solutions and/or possibly different 
probes on genomic Southerns first, then take what I learned to the DNaseI assay.  There are lots and 
lots of different recipies for hyb mixes out there -- it may be that yours gives high background for 
some reason.  Alternatively, your probe may have some repetitve elements in it which give the 
background smear. 

John Watson
Bristol-Myers Squibb Co.
watson_j at
"If you're not part of the solution, you're part of the precipitate."

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