help, buffer solution

Luis Lopes da Fonseca llfonseca at mail.telepac.pt
Fri Sep 6 01:05:51 EST 1996


I have been asked the following questions, that i am not able to
answer.

How would you select a buffer solution, if you wanted to:

a) purify an enzime
b) characterize kineticly an enzime associated 
   with the glicolitic pathway
c) determine the enzimatic activity of a NAD dependent dehydrogenase

and why (justify)?

I thank all the help.

Luis
llfonseca at mail.telepac.pt
Lisbon, Portugal



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