TUNEL and PI staining - apoptosis vs necrosis
apoptose at cortex.ama.ttuhsc.edu
Thu Sep 5 14:20:09 EST 1996
Cell morphology is the only definitive method.
TUNEl assay will tag any broken DNA strand, not only ones
from apoptotic cells.
Look for morphology characteristic of apoptotic cells:
ability to exclude vital dye such as trypan blue
Look for morphology characteristic of necrotic cells:
inability to exclude vital dye
Some use EM, but that is overkill.
Claire Payne, and Alison and Sarraf have the best reviews of morphology
(with pictures) Otherwise I can send examples.
More information about the Methods